DNA

Part:BBa_K4161502:Design

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-25)


A promoter that allow the plasmid to be expressed in S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter should be inducible in target S. cerevisiae strains. However, the expression of GAL1 should be repressed with the presence of glucose. Therefore, when trying to express the downstream region of this promoter the S. cerevisiae should be cultured in nutrient-deficit environments.


Source

GAL1 gene comes from S. cerevisiae chromosome 2.

References

Mateja Novak Štagoj, Aleksandra Comino, Radovan Komel, Fluorescence based assay of GAL system in yeast Saccharomyces cerevisiae, FEMS Microbiology Letters, Volume 244, Issue 1, March 2005, Pages 105–110, https://doi.org/10.1016/j.femsle.2005.01.041